Fig 1: MiR-92a-2-5p modulates the differentiation of ASD-NPCs. (A,B) Representative images of TUJ1 (green)-expressing neurons derived from ASD NC and ASD inhibitor NPCs at day 10. DAPI (blue) was used to stain nuclei. The percentage of TUJ1-labeled cells was calculated. (C,D) Representative images of MAP2 (green)-expressing neurons derived from ASD NC and ASD inhibitor NPCs at day 20. The percentage of MAP2-labeled cells was calculated. (E–G) Representative images of MAP2- (green) and VGAT (red)-expressing neurons derived from ASD NC and ASD-inhibitor NPCs at day 30. The percentage of MAP2- and VGAT-labeled cells was calculated. (H) Relative mRNA expression of SYP, PSD95, MAP2, and VGAT in ASD NC and ASD inhibitor NPCs at day 30, as determined by RT-qPCR. All data represent mean ± SEM; scale bar: 50 µm; *** p < 0.001, ** p < 0.01, * p < 0.05.